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alkaline phosphatase labeled goat anti mouse igg alp igg  (Bioss)
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Bioss alkaline phosphatase labeled goat anti mouse igg alp igg
Alkaline Phosphatase Labeled Goat Anti Mouse Igg Alp Igg, supplied by Bioss, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human alp  (R&D Systems)
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miR-468-3p is reduced during osteogenic differentiation. ( A ) BMSC characterization (flow cytometry). ( B ) MiR-468-3p levels in left femurs from ovariectomy (OVX) or sham mice at 6 weeks and 6 months old after surgery (OVX-6w and Sham-6w; 6 months old) (RT-PCR). ( C ) <t>ALP</t> and OC mRNA levels in left femurs after operations (RT-PCR). ( D ) MiR-214 levels (RT-PCR) in Alp + cells by fluorescence-activated cell <t>sorting</t> <t>(FACS)</t> from BMSCs in bilateral femurs from OVX- and sham-operated animals. ( E) OC mRNA levels (RT-PCR) in Alp + cells (FACS) from BMSCs in bilateral femurs from OVX- and sham-operated animals. Six mice/group. ( F ) BMSCs were treated with BMP-2 (200 ng/ml) or not for 4 days. Runx2, OC, and ALP levels by qRT-PCR. GAPDH = internal control. ( G ) BMSCs were treated using BMP-2 and miR-468-3p levels were assayed by qRT-PCR. U6 = internal control. Data are mean values ± SD. Studies were performed three times. * P < 0.05 compared with controls
Human Alp, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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alkaline phosphatase alp conjugated goat anti mouse igg  (Bio-Rad)
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Bio-Rad alkaline phosphatase alp conjugated goat anti mouse igg
miR-468-3p is reduced during osteogenic differentiation. ( A ) BMSC characterization (flow cytometry). ( B ) MiR-468-3p levels in left femurs from ovariectomy (OVX) or sham mice at 6 weeks and 6 months old after surgery (OVX-6w and Sham-6w; 6 months old) (RT-PCR). ( C ) <t>ALP</t> and OC mRNA levels in left femurs after operations (RT-PCR). ( D ) MiR-214 levels (RT-PCR) in Alp + cells by fluorescence-activated cell <t>sorting</t> <t>(FACS)</t> from BMSCs in bilateral femurs from OVX- and sham-operated animals. ( E) OC mRNA levels (RT-PCR) in Alp + cells (FACS) from BMSCs in bilateral femurs from OVX- and sham-operated animals. Six mice/group. ( F ) BMSCs were treated with BMP-2 (200 ng/ml) or not for 4 days. Runx2, OC, and ALP levels by qRT-PCR. GAPDH = internal control. ( G ) BMSCs were treated using BMP-2 and miR-468-3p levels were assayed by qRT-PCR. U6 = internal control. Data are mean values ± SD. Studies were performed three times. * P < 0.05 compared with controls
Alkaline Phosphatase Alp Conjugated Goat Anti Mouse Igg, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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goat anti-mouse igg, alp  (Mabtech Inc)
90
Mabtech Inc goat anti-mouse igg, alp
miR-468-3p is reduced during osteogenic differentiation. ( A ) BMSC characterization (flow cytometry). ( B ) MiR-468-3p levels in left femurs from ovariectomy (OVX) or sham mice at 6 weeks and 6 months old after surgery (OVX-6w and Sham-6w; 6 months old) (RT-PCR). ( C ) <t>ALP</t> and OC mRNA levels in left femurs after operations (RT-PCR). ( D ) MiR-214 levels (RT-PCR) in Alp + cells by fluorescence-activated cell <t>sorting</t> <t>(FACS)</t> from BMSCs in bilateral femurs from OVX- and sham-operated animals. ( E) OC mRNA levels (RT-PCR) in Alp + cells (FACS) from BMSCs in bilateral femurs from OVX- and sham-operated animals. Six mice/group. ( F ) BMSCs were treated with BMP-2 (200 ng/ml) or not for 4 days. Runx2, OC, and ALP levels by qRT-PCR. GAPDH = internal control. ( G ) BMSCs were treated using BMP-2 and miR-468-3p levels were assayed by qRT-PCR. U6 = internal control. Data are mean values ± SD. Studies were performed three times. * P < 0.05 compared with controls
Goat Anti Mouse Igg, Alp, supplied by Mabtech Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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goat anti-mouse igg, alkaline phosphatase (alp) antibodies  (Mabtech Inc)
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Mabtech Inc goat anti-mouse igg, alkaline phosphatase (alp) antibodies
miR-468-3p is reduced during osteogenic differentiation. ( A ) BMSC characterization (flow cytometry). ( B ) MiR-468-3p levels in left femurs from ovariectomy (OVX) or sham mice at 6 weeks and 6 months old after surgery (OVX-6w and Sham-6w; 6 months old) (RT-PCR). ( C ) <t>ALP</t> and OC mRNA levels in left femurs after operations (RT-PCR). ( D ) MiR-214 levels (RT-PCR) in Alp + cells by fluorescence-activated cell <t>sorting</t> <t>(FACS)</t> from BMSCs in bilateral femurs from OVX- and sham-operated animals. ( E) OC mRNA levels (RT-PCR) in Alp + cells (FACS) from BMSCs in bilateral femurs from OVX- and sham-operated animals. Six mice/group. ( F ) BMSCs were treated with BMP-2 (200 ng/ml) or not for 4 days. Runx2, OC, and ALP levels by qRT-PCR. GAPDH = internal control. ( G ) BMSCs were treated using BMP-2 and miR-468-3p levels were assayed by qRT-PCR. U6 = internal control. Data are mean values ± SD. Studies were performed three times. * P < 0.05 compared with controls
Goat Anti Mouse Igg, Alkaline Phosphatase (Alp) Antibodies, supplied by Mabtech Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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alp goat anti mouse igg  (Jackson Immuno)
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Jackson Immuno alp goat anti mouse igg
miR-468-3p is reduced during osteogenic differentiation. ( A ) BMSC characterization (flow cytometry). ( B ) MiR-468-3p levels in left femurs from ovariectomy (OVX) or sham mice at 6 weeks and 6 months old after surgery (OVX-6w and Sham-6w; 6 months old) (RT-PCR). ( C ) <t>ALP</t> and OC mRNA levels in left femurs after operations (RT-PCR). ( D ) MiR-214 levels (RT-PCR) in Alp + cells by fluorescence-activated cell <t>sorting</t> <t>(FACS)</t> from BMSCs in bilateral femurs from OVX- and sham-operated animals. ( E) OC mRNA levels (RT-PCR) in Alp + cells (FACS) from BMSCs in bilateral femurs from OVX- and sham-operated animals. Six mice/group. ( F ) BMSCs were treated with BMP-2 (200 ng/ml) or not for 4 days. Runx2, OC, and ALP levels by qRT-PCR. GAPDH = internal control. ( G ) BMSCs were treated using BMP-2 and miR-468-3p levels were assayed by qRT-PCR. U6 = internal control. Data are mean values ± SD. Studies were performed three times. * P < 0.05 compared with controls
Alp Goat Anti Mouse Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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alp-conjugated rabbit anti-mouse igg alp-ab2  (Beijing Solarbio Science)
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Beijing Solarbio Science alp-conjugated rabbit anti-mouse igg alp-ab2
miR-468-3p is reduced during osteogenic differentiation. ( A ) BMSC characterization (flow cytometry). ( B ) MiR-468-3p levels in left femurs from ovariectomy (OVX) or sham mice at 6 weeks and 6 months old after surgery (OVX-6w and Sham-6w; 6 months old) (RT-PCR). ( C ) <t>ALP</t> and OC mRNA levels in left femurs after operations (RT-PCR). ( D ) MiR-214 levels (RT-PCR) in Alp + cells by fluorescence-activated cell <t>sorting</t> <t>(FACS)</t> from BMSCs in bilateral femurs from OVX- and sham-operated animals. ( E) OC mRNA levels (RT-PCR) in Alp + cells (FACS) from BMSCs in bilateral femurs from OVX- and sham-operated animals. Six mice/group. ( F ) BMSCs were treated with BMP-2 (200 ng/ml) or not for 4 days. Runx2, OC, and ALP levels by qRT-PCR. GAPDH = internal control. ( G ) BMSCs were treated using BMP-2 and miR-468-3p levels were assayed by qRT-PCR. U6 = internal control. Data are mean values ± SD. Studies were performed three times. * P < 0.05 compared with controls
Alp Conjugated Rabbit Anti Mouse Igg Alp Ab2, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse monoclonal anti-alp  (Thermo Fisher)
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Thermo Fisher mouse monoclonal anti-alp
miR-468-3p is reduced during osteogenic differentiation. ( A ) BMSC characterization (flow cytometry). ( B ) MiR-468-3p levels in left femurs from ovariectomy (OVX) or sham mice at 6 weeks and 6 months old after surgery (OVX-6w and Sham-6w; 6 months old) (RT-PCR). ( C ) <t>ALP</t> and OC mRNA levels in left femurs after operations (RT-PCR). ( D ) MiR-214 levels (RT-PCR) in Alp + cells by fluorescence-activated cell <t>sorting</t> <t>(FACS)</t> from BMSCs in bilateral femurs from OVX- and sham-operated animals. ( E) OC mRNA levels (RT-PCR) in Alp + cells (FACS) from BMSCs in bilateral femurs from OVX- and sham-operated animals. Six mice/group. ( F ) BMSCs were treated with BMP-2 (200 ng/ml) or not for 4 days. Runx2, OC, and ALP levels by qRT-PCR. GAPDH = internal control. ( G ) BMSCs were treated using BMP-2 and miR-468-3p levels were assayed by qRT-PCR. U6 = internal control. Data are mean values ± SD. Studies were performed three times. * P < 0.05 compared with controls
Mouse Monoclonal Anti Alp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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alp labeled goat antimouse igg  (Jackson Immuno)
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Jackson Immuno alp labeled goat antimouse igg
miR-468-3p is reduced during osteogenic differentiation. ( A ) BMSC characterization (flow cytometry). ( B ) MiR-468-3p levels in left femurs from ovariectomy (OVX) or sham mice at 6 weeks and 6 months old after surgery (OVX-6w and Sham-6w; 6 months old) (RT-PCR). ( C ) <t>ALP</t> and OC mRNA levels in left femurs after operations (RT-PCR). ( D ) MiR-214 levels (RT-PCR) in Alp + cells by fluorescence-activated cell <t>sorting</t> <t>(FACS)</t> from BMSCs in bilateral femurs from OVX- and sham-operated animals. ( E) OC mRNA levels (RT-PCR) in Alp + cells (FACS) from BMSCs in bilateral femurs from OVX- and sham-operated animals. Six mice/group. ( F ) BMSCs were treated with BMP-2 (200 ng/ml) or not for 4 days. Runx2, OC, and ALP levels by qRT-PCR. GAPDH = internal control. ( G ) BMSCs were treated using BMP-2 and miR-468-3p levels were assayed by qRT-PCR. U6 = internal control. Data are mean values ± SD. Studies were performed three times. * P < 0.05 compared with controls
Alp Labeled Goat Antimouse Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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alp labeled goat anti mouse igg  (SouthernBiotech)
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SouthernBiotech alp labeled goat anti mouse igg
miR-468-3p is reduced during osteogenic differentiation. ( A ) BMSC characterization (flow cytometry). ( B ) MiR-468-3p levels in left femurs from ovariectomy (OVX) or sham mice at 6 weeks and 6 months old after surgery (OVX-6w and Sham-6w; 6 months old) (RT-PCR). ( C ) <t>ALP</t> and OC mRNA levels in left femurs after operations (RT-PCR). ( D ) MiR-214 levels (RT-PCR) in Alp + cells by fluorescence-activated cell <t>sorting</t> <t>(FACS)</t> from BMSCs in bilateral femurs from OVX- and sham-operated animals. ( E) OC mRNA levels (RT-PCR) in Alp + cells (FACS) from BMSCs in bilateral femurs from OVX- and sham-operated animals. Six mice/group. ( F ) BMSCs were treated with BMP-2 (200 ng/ml) or not for 4 days. Runx2, OC, and ALP levels by qRT-PCR. GAPDH = internal control. ( G ) BMSCs were treated using BMP-2 and miR-468-3p levels were assayed by qRT-PCR. U6 = internal control. Data are mean values ± SD. Studies were performed three times. * P < 0.05 compared with controls
Alp Labeled Goat Anti Mouse Igg, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


miR-468-3p is reduced during osteogenic differentiation. ( A ) BMSC characterization (flow cytometry). ( B ) MiR-468-3p levels in left femurs from ovariectomy (OVX) or sham mice at 6 weeks and 6 months old after surgery (OVX-6w and Sham-6w; 6 months old) (RT-PCR). ( C ) ALP and OC mRNA levels in left femurs after operations (RT-PCR). ( D ) MiR-214 levels (RT-PCR) in Alp + cells by fluorescence-activated cell sorting (FACS) from BMSCs in bilateral femurs from OVX- and sham-operated animals. ( E) OC mRNA levels (RT-PCR) in Alp + cells (FACS) from BMSCs in bilateral femurs from OVX- and sham-operated animals. Six mice/group. ( F ) BMSCs were treated with BMP-2 (200 ng/ml) or not for 4 days. Runx2, OC, and ALP levels by qRT-PCR. GAPDH = internal control. ( G ) BMSCs were treated using BMP-2 and miR-468-3p levels were assayed by qRT-PCR. U6 = internal control. Data are mean values ± SD. Studies were performed three times. * P < 0.05 compared with controls

Journal: Journal of Orthopaedic Surgery and Research

Article Title: miR-468-3p suppresses osteogenic differentiation of BMSCs by targeting Runx2 and inhibits bone formation

doi: 10.1186/s13018-024-05410-7

Figure Lengend Snippet: miR-468-3p is reduced during osteogenic differentiation. ( A ) BMSC characterization (flow cytometry). ( B ) MiR-468-3p levels in left femurs from ovariectomy (OVX) or sham mice at 6 weeks and 6 months old after surgery (OVX-6w and Sham-6w; 6 months old) (RT-PCR). ( C ) ALP and OC mRNA levels in left femurs after operations (RT-PCR). ( D ) MiR-214 levels (RT-PCR) in Alp + cells by fluorescence-activated cell sorting (FACS) from BMSCs in bilateral femurs from OVX- and sham-operated animals. ( E) OC mRNA levels (RT-PCR) in Alp + cells (FACS) from BMSCs in bilateral femurs from OVX- and sham-operated animals. Six mice/group. ( F ) BMSCs were treated with BMP-2 (200 ng/ml) or not for 4 days. Runx2, OC, and ALP levels by qRT-PCR. GAPDH = internal control. ( G ) BMSCs were treated using BMP-2 and miR-468-3p levels were assayed by qRT-PCR. U6 = internal control. Data are mean values ± SD. Studies were performed three times. * P < 0.05 compared with controls

Article Snippet: The femurs of mice were used to collect stem cells from bone marrow, which were used in FACS analyses with PerCP-labeled antibodies to rat, mouse, and human ALP (R&D Systems, AF2910, 1:50).

Techniques: Flow Cytometry, Reverse Transcription Polymerase Chain Reaction, Fluorescence, FACS, Quantitative RT-PCR, Control